menH [2019-07-01 09:25:53]

menaquinone biosynthesis methyltransferase

Locus: BSU22750

Isoelectric point: 8.17

Molecular weight: 26.43 kDa

Protein length: 233 aa

Gene length: 702 bp

Function: biosynthesis of menaquinone

Product: menaquinone biosynthesis methyltransferase

Essential: no

E.C.: 2.1.1.163

Synonyms: gerCB,menG

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of menaquinone
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for biofilm formation

Gene

Coordinates: 2,382,907 → 2,383,608

Phenotypes of a mutant

• essential according to Kobayashi et al. ; however, inactivation reported by Meeske et al. and Block et al.
• inactivation of menH reduces sporulation efficiency to 12.7% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• S-adenosyl-L-methionine + demethylmenaquinol = s-adenosyl-L-homocysteine + menaquinol (according to Swiss-Prot)

Protein family

• MenG/UbiE family (single member, according to UniProt)

Structure

• 1OBW (from Saccharomyces cerevisiae, 31% identity) PubMed

Expression and Regulation

Operons

• Genes: hepS-menH-hepT-ndk
  Description: PubMed

Biological materials

Mutant

• BKE22750 (ΔmenH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA, downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT
• BKK22750 (ΔmenH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA, downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT

References

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